Chromosome conformation by crosslinking
نویسندگان
چکیده
منابع مشابه
Chromosome conformation by crosslinking: polymer physics matters.
The genetic information is encoded on double-stranded DNA, a long linear polymer chain. Among the central themes of Nucleus will be the advancement of our understanding of how those chains are folded so that they fit into the cell nucleus, and at the same time their information can be read off efficiently. In fact, a quantitative description of the structure of the folded genome is one of the m...
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Complex I is the only component of the eukaryotic respiratory chain of which no high-resolution structure is yet available. A notable feature of mitochondrial complex I is the so-called active/de-active conformational transition of the idle enzyme from the active (A) to the de-active, (D) form. Using an amine- and sulfhydryl-reactive crosslinker of 6.8Å length (SPDP) we found that in the D-form...
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Chemical cross-linking and DNA sequencing have revealed regions of intra-chromosomal interaction, referred to as topologically associating domains (TADs), interspersed with regions of little or no interaction, in interphase nuclei. We find that TADs and the regions between them correspond with the bands and interbands of polytene chromosomes of Drosophila. We further establish the conservation ...
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ژورنال
عنوان ژورنال: Nucleus
سال: 2010
ISSN: 1949-1034,1949-1042
DOI: 10.4161/nucl.1.1.10837